Probe-based qPCR: Probe Compatibility and Multiplexing with Luna Universal Probe qPCR Master Mix
نویسندگان
چکیده
Quantitative PCR (qPCR) is a commonly used molecular biology technique that enables the precise determination of the amount of DNA or RNA in a sample of interest. By measuring the amount of DNA present at each cycle of a PCR, the concentration in an unknown sample can be calculated by comparison to a set of known standard samples. This real-time measurement of the PCR product is achieved using fluorescent reporters, broadly grouped into two classes:
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